Figure 11 of Wiechmann, Mol Vis 2009; 15:2384-2403.

Figure 11. Confocal analysis of Mel1a and Mel1c immunocytochemistry of whole-mounted Xenopus laevis surface corneal epithelium at two separate time points. Three-dimensional reconstructions of confocal z-stacks of optical slices of the 8:00 AM (A, C, and E) and 4:00 PM (B, D, and F) specimens were rotated at 63° on the x-axis to enable optimal viewing of the pattern of immunolabeling. At 8:00 AM (A, C, and E), areas of lateral membranes express only the red Mel1a receptor label (large arrow) or the green Mel1c receptor label (large arrowhead). In some areas of merged Mel1a­-Mel1c co-localization, only the yellow color is observed (small arrow), indicating receptor co-localization. There are also areas of membrane that express the yellow co-localization but have small areas of red Mel1a or green Mel1c label interdigitated with the yellow label (asterisks). At 4:00 PM (B, D, and F), the lateral membranes show a more uniform pattern of immunolabeling than observed at the 8:00 AM time point. The yellow co-localization label is predominant in the most apical portion of the membranes, but in the more basal area of the lateral membranes distinct punctate red Mel1a and green Mel1c labeling is also observed. Nuclei are stained with DAPI. The confocal images in panels A and E are comprised of 16 optical slices of 400 nm each in the z-series. The confocal images in panels B and F are comprised of 16 optical slices of 400 nm each in the z-series. The images in panels C and D are comprised of a single optical slice of 400 nm. The magnification bar (F) represents 20 µm.