Table 2 of Huang, Mol Vis 2009; 15:2358-2363.

Table 2. Thermodynamic parameters of goose δ-crystallin unfolded in Gdm-HCl


Proteins		 ΔG1o ΔG2o ΔG3o ΔGto [D]11/2 [D]21/2 [D]31/2
(kcal/mol) (M)
WT 7.7±0.8 3.4±0.8 8.2±1.8 19.3 1.0±0.1 2.1±0.5 4.1±0.8
E294L 5.7±0.5 1.8±0.5 4.3±0.7 11.8 0.5±0.1 1.5±0.4 3.9±0.6

[D]1/2 is the half concentration of Gdm-HCl required in the unfolding transition of δ–crystallin. ΔGto is the summation of ΔG1o, ΔG2o, and ΔG3o. These values were derived from Figure 2B after global fitting the unfolding curve to the equation based on a 4-state model [15].

Huang, Mol Vis 2009; 15:2358-2363. http://www.molvis.org/molvis/v15/a252

©2009 Molecular Vision http://www.molvis.org/molvis/

ISSN 1090-0535