Figure 7. Immunofluorescent staining of MIF in corneal and conjunctival epithelia. Immunofluorescence staining demonstrated the presence of MIF (red) in corneal epithelia (CE: arrows) and conjunctival epithelia (CJE: arrow heads) at all time points. The staining was detected in normal (A, E, I, and M) and BTX-B injected mice (B, F, J, and N) and saline-injected mice (D, H, L, and P) and showed no difference (C, G, K, and O). Negative control with no staining. Isotype control was omitted with no staining. Scale bar: 50 μm.