Figure 5. Immunofluorescent staining with TNF-α specific antibody in corneal and conjunctival epithelia. Immunofluorescence staining with TNF- α specific antibody (green) in corneal epithelia (CE: arrows) and conjunctival epithelia (CJE: arrow heads) at all time points. The staining for TNF-α localized in CE and CJE was strong in BTX-B injected mice. Nuclear staining (blue). (F-J) and (P-T): merged pictures. Images of isotype and negative control were omitted with no staining. Scale bar: 50 μm.