Molecular Vision: Greiling, Mol Vis 2009; 15:2313-2325. Table 3

Table 3. The change in γ-crystallin proteins with age was determined in zebrafish lenses by shotgun proteomics analysis.

Crystallin Protein	Rank order (relative abundance)				Chromosome	IPI
Crystallin Protein	4.5 days	3 weeks	6 weeks	6 months	Chromosome	IPI
γM1	-	32	32	14	9	IPI00495938.1
zgc:153846 (“γM1b”)	25	20	21	62	9	IPI00607433.4
γM2a	-	19	10	23	9	IPI00607295.1
γM2b	-	17	35	18	9	IPI00504980.1
γM2c	48	-	105	12	9	IPI00503886.1
γM2d1	-	-	-	206	9	IPI00485200.3
γM2d2	-	21	51	-	9	IPI00505178.4
γM2d5	-	-	-	185	9	IPI00614258.2
γM2d6	-	37	42	-	9	IPI00638856.2
γM2d7	93	99	122	-	9	IPI00486384.5
γM2d8	17	16	20	-	9	IPI00487422.2
LOC799807 (“γM2d9”)	15	-	52	-	9	IPI00835330.1
zgc:171758 (“γM2d10”)	26	29	40	74	9	IPI00859358.1
zgc:171793 (“γM2d11”)	-	40	37	-	9	IPI00866205.1
zgc:171791 (“γM2d12”)	12	11	12	226	9	IPI00833949.1
zgc:92692 (“γM2d13”)	13	12	17	108	9	IPI00502160.1
zgc:171792 (“γM2d14”)	16	-	24	-	9	IPI00863220.1
zgc:92724 (“γM2d15”)	18	25	29	63	9	IPI00774533.1
zgc:173495 (“γM2d16”)	-	74	95	-	9	IPI00866651.1
LOC569604 (“γM2e”)	-	15	11	46	9	IPI00613116.2
zgc:172241 (“γM2f”)	-	26	18	173	9	IPI00489442.3
γM3	-	84	22	21	9	IPI00607324.4
γM4	-	38	31	22	21	IPI00485316.1
γM5	-	-	47	41	9	IPI00483712.2
γM6	-	103	43	85	9	IPI00507423.3
γM7	-	68	28	27	9	IPI00509894.2
γMX	11	5	6	7	12	IPI00864931.1
zgc:110028 (“γMXb”)	-	88	48	166	9	IPI00503899.2
zgc:110021 (“γMXc”)	6	7	19	35	12	IPI00607474.1
γN1	-	27	14	13	2	IPI00499329.1
γN2	5	3	5	29	24	IPI00495773.1
γS1	-	-	93	3	22	IPI00495605.2
γS2	-	-	-	15	9	IPI00868287.1
γS3	-	-	-	28	9	IPI00500990.2
γS4	-	-	98	16	9	IPI00486227.2

The numbers in columns 2–5 represent the rank order of protein abundance at each age listed (i.e., “1” indicates the most abundant protein detected in the lens). Column 6 “Chromosome” lists the chromosome from which the corresponding gene is transcribed. Out of the total 36 γ-crystallin proteins identified, only 13 were detected at 4.5 days and 27 were detected at six months, indicating an increase in γ-crystallin abundance and diversity with aging. Thirteen novel γ-crystallins were identified. IPI refers to the International Protein Index reference number.