Molecular Vision: Greiling, Mol Vis 2009; 15:2313-2325. Table 2

Table 2. The change in α- and β-crystallin proteins with age was determined in zebrafish lenses by shotgun proteomics analysis.

Crystallin protein	Rank order (relative abundance)				Chromosome	IPI
Crystallin protein	4.5 days	3 weeks	6 weeks	6 months	Chromosome	IPI
αA	28	28	13	1	1	IPI00509939.2
αBa	-	-	-	9	15	IPI00482033.2
αBb	-	-	80	26	5	IPI00488884.1
βA1a	-	-	27	177	15	IPI00502528.2
βA1b	10	10	4	25	21	IPI00503999.3
βA2–1	7	14	9	11	6	IPI00495820.1
βA2–2	8	9	8	8	9	IPI00513173.2
βA4	9	8	1	10	19	IPI00490966.2
βB1	1	1	-	5	10	IPI00502990.4
βB2	-	24	15	4	8	IPI00501506.3
βB3	-	-	54	2	5	IPI00607344.1
βγX	83	-	71	82	7	IPI00493885.5
βA1c predicted (“βA1c”)	63	36	26	20	1	IPI00503128.2
βA1 crystallin, like (“βA1d”)”	4	6	7	32	14	IPI00504818.3
LOC553473 (“βB1b”)	2	2	2	57	14	IPI00607401.3
zgc:171773 (“βB1c”)	3	4	3	6	1	IPI00859087.1
zgc:171636 (“βB1d”)	-	-	76	80	15	IPI00858800.1

The numbers in columns 2–5 represent the rank order of protein abundance at each age listed (i.e., “1” indicates the most abundant protein detected in the lens). Column 6 “Chromosome” lists the chromosome from which the corresponding gene is transcribed. All three α-crystallins increased in abundance during lens maturation. β-Crystallins were abundant at all ages examined, and five novel β-crystallin proteins were identified. IPI refers to the International Protein Index reference number.