Figure 4. Detection of MMP-2 and MMP-9 by gelatin zymography. Treatment with pioglitazone at concentrations of 1, 3, and 10 μm resulted in significant decreases in MMP-2 and MMP-9 secretion. A: A representative zymogram is shown. Lane 1, control; lane 2, 1 μm; lane 3, 3 μm; lane 4, 10 μm. B: The densitometric values of the MMP-9 and MMP-2 bands are expressed as percentages of the control. Results are the mean±SD of three independent experiments (the asterisk indicates p<0.05 versus control), indicating a dose-dependent inhibition of pioglitazone on MMP-9 and MMP-2 secretion.