Figure 1. Expression of α-smooth muscle
actin in corneal myofibroblasts. The third passage corneal fibroblasts
cultured on coverslips were washed with PBS, fixed with 4%
paraformaldehyde, blocked in BSA/PBS, and sequentially incubated with
monoclonal α-smooth muscle actin and FITC-conjugated secondary
antibody. The nuclei were counterstained with
4'-6-diamidino-2-phenylindole(DAPI). Negative controls were performed
using nonimmune serum instead of primary antibodies. A:
Co-localization of α-SMA (green) and nuclei (blue) in myofibroblasts. B:
Negative control.