Figure 2 of Giove, Mol Vis 2009; 15:2249-2258.


Figure 2. nNOS protein levels decreased after 5 weeks of diabetes. A: Mean fluorescence levels of nNOS immunoreactivity were significantly reduced in the outerplexiform layer (OPL), inner nuclear layer (INL), inner plexiform layer (IPL), and ganglion cell layer (GCL) when compared to controls (n=11). The graph on the right indicates the line profile average of the intensity of nNOS in each layer of the retina. B: nNOS immunoreactivity in the IPL no longer filled neuronal processes in the diabetic and was localized in structures which resembled synaptic boutons in size and location. C: western blots detected a single ~160 kDa band consistent with nNOS. Levels of β-tubulin are shown to confirm equal protein loading. There was a 24%±11 decrease in total nNOS protein (C is control, D is diabetic; n=8). Line on the left indicates the location of the 150 kDa molecular weight marker.