Figure 4. Gradual increase in proliferative potential according to the amniotic membrane concentration in human corneal epithelial cells
monolayers. 5-Bromo-2-deoxyuridine (BrdU) labeling (green) in the nucleus (blue, stained with 4',6-diamidino-2-phenylindole
[DAPI]) was conducted to evaluate the proliferative potential of human corneal epithelial cells monolayers cultured at 0%
amniotic membrane (A), 5% AM (B), 30% AM (C), and that of the positive control (D). E: Statistically significant increases in proliferative potential, as compared to the negative controls, were noted in the
30% AM and positive control groups (asterisk; N=3; Scale bar, 200 µm).