Figure 3. Western blot analysis of proliferating cell nuclear antigen, E-cadherin, Fibronectin-1, and MMP9 The results are representative of three independent experiments. Western blot and densitometric analyses demonstrated that HCECs exposed to AM (5% and 30%) evidenced increased levels of PCNA and MMP9 protein (p<0.05 and p<0.001, respectively), whereas HCECs exposed to AM (5% and 30%) exhibited reduced expression levels of E-cadherin and Fibronectin-1 (p<0.05 and p<0.001, respectively). The values displayed in these graphs are expressed as the means±SD of triplicate determinations in a representative experiment. Values were normalized to that of the actin expression.