Figure 2. The effects of amniotic membrane suspensions on E-cadherin, Fibronectin-1, and MMP9 in corneal epithelial cells human corneal epithelial cells. RT-PCR and densitometric analyses demonstrated that human corneal epithelial cells exposed to amniotic membrane suspensions (0%, 5%, and 30%), and the positive controls (SP) evidenced increased levels of MMP9 mRNA (p<0.05 in 5% and 30% AM, and SP). On the other hand, the expressions of E-cadherin and Fibronectin-1 were reduced in accordance with the AM concentration. The values displayed in these graphs are expressed as the means±SD of triplicate determinations in a representative experiment. PCR experiments were normalized to the levels of GAPDH expression.