Figure 8 of Takata, Mol Vis 2009; 15:241-249.


Figure 8. Thermal stability of β-crystallin complexes. Samples were heated at 55 °C and the turbidity of the solutions measured at 405 nm. βB1- and βB2-crystallin were mixed with βA3 WT (A) or βA3 DM (B). As a control, βA3 DM was also mixed with the known chaperone, αA-crystallin (B). In order to determine if either βB1 or βB2 had chaperone-like activity, the substrate alcohol dehydrogenase (ALDH) was used (C). Both βB1 and βB2 decreased the heat induced precipitation of βA3 WT, but only βB1 decreased the precipitation of βA3 DM. Neither βB1 nor βB2 prevented the precipitation of ALDH.