Figure 8. Thermal stability of
β-crystallin complexes. Samples were heated at 55 °C and the turbidity
of the solutions measured at 405 nm. βB1- and βB2-crystallin were mixed
with βA3 WT (A) or βA3 DM (B). As a control, βA3 DM was
also mixed with the known chaperone, αA-crystallin (B). In order
to determine if either βB1 or βB2 had chaperone-like activity, the
substrate alcohol dehydrogenase (ALDH) was used (C). Both βB1
and βB2 decreased the heat induced precipitation of βA3 WT, but only
βB1 decreased the precipitation of βA3 DM. Neither βB1 nor βB2
prevented the precipitation of ALDH.