Figure 1. Purified recombinant crystallins. Recombinantly expressed proteins were purified to near homogeneity as indicated by a single band on SDS-PAGE. Each protein (1 μg) was visualized with Coomassie blue stain on a 1.0 mm thick, 4-12 % Bis/Tris gel. Proteins were βB1 (lane 1), βB2 (lane 2), βA3 WT (lane 3), βA3 DM (lane 4) and αA-crystallin (lane 5). Triangles indicate positions of molecular weight markers.