Table 1 of Cao, Mol Vis 2009; 15:2202-2208. Table 1 of Cao, Mol Vis 2009; 15:2202-2208.
Table 1. Primer pairs and enzymes used for CALCRL tag snp genotyping.
dbSNP ID |
Forward primer |
Reverse primer |
Product length (bp) |
Tm (°C) |
Restriction enzyme |
|---|---|---|---|---|---|
| rs7591567 | GTCACGCTGAGGTAGG | GATTTCATTTGCCACC | 485 | 56 | BstZ171 |
| rs3771073 | CAGAATGTAGCAGGAC | CTCTAAAGGGATGGTT | 215 | 51 | Ddel |
| rs3821181 | GATACCTAGGGAACCA | ACAACAGCGAAACATT | 280 | 51 | AflII |
| rs9288141 | CCTGCAAGACAATCCC | ATCCGACAAGGTGAGC | 381 | 56.6 | HpaII |
| rs1157699 | GATGAAAGCCTGAGAA | ACCTGCCTCCATACTC | 165 | 52.4 | BanII |
| rs6759535 | TTTATTGAGTGCCTAC | AAATGGACCATGTTTA | 381 | 50.3 | HpaII |
| rs840617 | TCCATTGGCTAAGTCC | CCCTTACCCTTTCCAG | 353 | 54.3 | HpyCH4IV |