Table 2 of Martínez-Osorio, Mol Vis 2009; 15:2185-2195.

Table 2. Reagents for flow cytometry.

Reagent Specificity Channel*
CK7-FITC mAb (clone LP5K) Secretory epithelium FL-1
CD45-PC7 mAb (clone J33) Peripheral blood leukocytes, APC and PMN cells FL-5
AnnexinV-FITC Phosphatidylserine, which is translocated from the inner to the outer leaflet of the cell membrane in early apoptosis process FL-1
PI in apoptosis assay DNA of cells with permeable membranes FL-3
PI in cell cycle assay DNA of cells in which membranes were permeabilized in the protocol FL-3
IgG2b-FITC mAb Isotype negative control FL-1
IgG1- PC7 mAb Isotype negative control FL-5

CK7: Cytokeratin 7; CD45: Leukocyte common antigen; mAb: Monoclonal antibody; FITC: Fluorescein isothiocyanate; PC7: Phycoerythrin-cyanin 7; APC: Antigen-presenting cell; PMN: Polymorphonuclear; PI: Propidium iodide. The asterisk indicates that each channel reads a specific fluorochrome: FL-1, FITC; FL-3, PI; FL-5, PC7.

Martínez-Osorio, Mol Vis 2009; 15:2185-2195. http://www.molvis.org/molvis/v15/a235

©2009 Molecular Vision http://www.molvis.org/molvis/

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