Table 2 of Martínez-Osorio, Mol Vis 2009; 15:2185-2195.


Table 2. Reagents for flow cytometry.

Reagent Specificity Channel*
CK7-FITC mAb (clone LP5K) Secretory epithelium FL-1
CD45-PC7 mAb (clone J33) Peripheral blood leukocytes, APC and PMN cells FL-5
AnnexinV-FITC Phosphatidylserine, which is translocated from the inner to the outer leaflet of the cell membrane in early apoptosis process FL-1
PI in apoptosis assay DNA of cells with permeable membranes FL-3
PI in cell cycle assay DNA of cells in which membranes were permeabilized in the protocol FL-3
IgG2b-FITC mAb Isotype negative control FL-1
IgG1- PC7 mAb Isotype negative control FL-5