Table 2 of Martínez-Osorio, Mol Vis 2009; 15:2185-2195.
Reagent | Specificity | Channel* |
---|---|---|
CK7-FITC mAb (clone LP5K) | Secretory epithelium | FL-1 |
CD45-PC7 mAb (clone J33) | Peripheral blood leukocytes, APC and PMN cells | FL-5 |
AnnexinV-FITC | Phosphatidylserine, which is translocated from the inner to the outer leaflet of the cell membrane in early apoptosis process | FL-1 |
PI in apoptosis assay | DNA of cells with permeable membranes | FL-3 |
PI in cell cycle assay | DNA of cells in which membranes were permeabilized in the protocol | FL-3 |
IgG2b-FITC mAb | Isotype negative control | FL-1 |
IgG1- PC7 mAb | Isotype negative control | FL-5 |