Figure 5. Effect of substratum on cell viability. On Day 5 of tarsal conjunctival cell culture in 10% fetal bovine serum (FBS) supplemented medium (FBSm10), the calcein dye was retained within living cells (green) while EthD-III was bound to nucleic acids of cells with damaged membranes (red). A: Numerous epithelial cells grown on Permanox™ showed red fluorescence typical of dead or dying cells. B: In contrast, cells grown on denuded amniotic membrane (dAM) showed less red fluorescence. Scale bar 50 μm (A) and 25 μm (B).