Figure 2 of Martínez-Osorio, Mol Vis 2009; 15:2185-2195.

Figure 2. Tarsal conjunctival cell viability, apoptosis, and cell death determined by flow cytometry. A: The morphological characteristics of recovered tarsal cells defined two populations: larger, more complex cells (red) and smaller, less complex cells (blue). Side scatter/forward scatter was used to define the size/complexity of the cell pool. B: In this representative experiment, early apoptotic cells (J4) and late apoptotic cells (J2) composed 70.9% of the population of larger, more complex cells stained with propidium iodide (PI) and annexin V, consistent with high level of apoptosis. C: In a population of smaller, less complex cells, 76.5% (D3) had negative staining with PI and annexin V, indicating high viability.