Figure 1 of Martínez-Osorio, Mol Vis 2009; 15:2185-2195.

Figure 1. Cell lineage analysis determined by flow cytometry. A: Forward scatter (FS) versus side scatter (SS) plot showing conjunctival tarsal cells recovered by brush cytology (BC) in 10% fetal bovine serum (FBS) supplemented medium (FBSm10). B: CD45 versus SS dot plot showing a leukocyte population (gate A, red), epithelial cells (gate B, blue) and cellular debris (gray dots). C: CD45 versus CK7 dot plot showing a CD45+CK7- leukocyte population (region L4), CK7+CD45- epithelial cells (region L1) and CK7-CD45- cellular debris (region L3). D: Analysis of CK7 expression (blue line) over total BC-recovered cells; 67.1% of the cells were positive (isotype-matched negative control in dotted line).