Table 1 of Kanda, Mol Vis 2009; 15:2174-2184.

Table 1. Subcellular localization of mutant NR2E3 proteins.

Amino
acid
change
Nuclear
(%)		 Nuclear and
cytoplasmic
(%)
Cytoplasmic
(%)
WT 97 2.7 0.3
p.G56R 95.3 3.7 1
p.N65_C67del 89.3 9 1.7
p.R76Q 56.7 29 14.3
p.R76W 52.5 26.8 20.7
p.G88V 90.5 6.3 3.2
p.R97H 59.5 29.2 11.3
p.R104Q 90.8 8 1.2
p.R104W 90.5 6 3.5
p.E121K 94.5 5 0.5
p.E140G 92.5 5.8 1.7
p.M163T 95.2 3.5 1.3
p.V232I 94.5 4.8 0.7
p.W234S 29.5 54.7 15.8
p.A256E 28.5 63.2 8.3
p.L263P 27.2 60.3 12.5
p.P276fs 13.5 79.7 6.8
p.V302I 92 6.2 1.8
p.R309G 83.5 15 1.5
p.R311Q 70.3 25.2 4.5
p.R334G 71.2 22.8 6
p.L336P 14.5 72 13.5
p.Q350X 5.5 80 14.5
p.L353V 5.5 74.2 20.3
p.R385P 35.2 58 6.8
p.M407K 38 59 3

Cellular localization of WT and mutant NR2E3 proteins was examined in COS-1 cells after transfection with the expression construct. Mutations affecting NR2E3 nuclear localization (p.R76Q, p.R76W, p.R97H, p.W234S, p.A256E, p.L263P, p.P276fs, p.R309G, p.R311Q, p.R334G, p.L336P, p.Q350X, p.L353V, p.R385P, and p.M407K) demonstrate increased cytoplasmic staining. More than 200 cells were scored from at least two independent immunocytochemistry experiments.

Kanda, Mol Vis 2009; 15:2174-2184. http://www.molvis.org/molvis/v15/a234

©2009 Molecular Vision http://www.molvis.org/molvis/

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