Figure 4. Interaction of WT and mutant NR2E3 proteins with NRL or CRX. A: Xpress-tagged WT or mutant NR2E3 expression plasmids were cotransfected into COS-1 cells with V5-tagged NRL expression construct. After immunoprecipitation with goat anti-V5 antibody, NR2E3 protein was detected with mouse anti-Xpress antibody. B: NR2E3 expression constructs were cotransfected into COS-1 cells with V5-tagged CRX expression construct. NR2E3 was visualized with mouse anti-Xpress antibody. In lower panels, the intensity of the WT NR2E3 immunoreactive band (normalized to input NR2E3) was compared with mutant NR2E3 proteins. ANOVA with a post hoc test were performed on each sample compared to WT NR2E3. Significant differences of p<0.05, and p<0.01 are shown as * and **, respectively.