Figure 2. Subcellular localization of WT and mutant NR2E3 proteins in COS-1 cells. COS-1 cells expressing WT or mutant NR2E3 proteins were incubated with anti-Xpress antibody (anti-Xp) and visualized using anti-mouse IgG-Alexa 488 antibody (green). Central panels show nuclei labeled with bisbenzimide (blue). The bottom panels display merged images. Scale bar represents 50 μm.