Figure 5 of Alge-Priglinger, Mol Vis 2009; 15:2162-2173.


Figure 5. Soluble galectin-1 reduces RPE cell migration on fibronectin and laminin in a dose-dependent manner. A, B: Boyden chamber membranes were coated with either fibronectin (Fn; A) or laminin (Lam; B). RPE suspensions were incubated for 35 min with the indicated concentrations of galectin-1 and then 5×104 cells were placed into the upper chamber. After five hours at 37 °C, cells that migrated into the lower surface of the filter were fixed, stained and quantified. Values indicate means±SD of four experiments performed in duplicate and are expressed as percentage of controls without galectin-1 in the medium. C: Galectin-1 as a substratum does not support RPE cell migration. Boyden chamber membranes were coated with either Fn alone, Fn+galectin-1 (Gal-1), Lam alone, Lam+Gal-1, or Gal-1 alone. RPE cells were then placed into the upper chamber at a density of 5×104 cells per chamber and allowed to migrate as described above. Values indicate means±SD of three experiments performed in duplicate and are expressed as percentage of controls (Co). Statistical analysis was performed using Students t-test and a p value <0.05 was considered as statistically significant (*). RPE cells migrating on uncoated filters served as controls (Co).