Figure 3 of Alge-Priglinger, Mol Vis 2009; 15:2162-2173.


Figure 3. Effect of galectin-1 on RPE cell adhesion. A, C: Galectin-1 inhibits RPE cell attachment to Fn (A) and Lam (C) in a dose-dependent manner. RPE suspensions were incubated for 35 min with the indicated concentrations of galectin-1 (Gal-1) and then plated on wells coated with Fn or Lam at a density of 2×104 cells per well. Values indicate means±SD of four experiments performed in triplicate and are expressed as percentage of controls without galectin-1 in the medium. B, D: The inhibitory effect of galectin-1 on attachment of RPE cells to Fn (B) and Lam (D) is mediated via carbohydrate binding. RPE cells were initially incubated in the presence or absence of 100 mM β-lactose (lac) as indicated. Values represent means±SD of three experiments performed in triplicate and are expressed as percentage of controls (Co) without galectin-1 or β-lactose in the medium. Statistical analysis was performed using Students t-test and a p value <0.05 was considered as statistically significant (*).