Figure 2. RPE cell viability was unaffected by galectin-1. Human RPE cells were seeded in 96-well plates at an initial density of 1×104 cells/well in DMEM supplemented with 10% FCS. Cells were cultured overnight before the indicated concentrations of galectin-1
were added to the medium in DMEM containing 2% FCS, and cell survival was assessed by MTT assay after 24 h (A) and 48 h (B) of culture. RPE cells cultured with incubation medium alone served as controls. The values were normalized to untreated
controls (100%) and represent the mean±SD of six experiments performed in duplicate wells.