Figure 3. Chromogenic in situ hybridization for chromosome 18 and 8q22 amplification in breast carcinoma. A: Invasive melanoma, hybridized with chromosome 18 showing two copies in all the cells. B: Non-metastasizing melanoma hybridized with chromosome 18 showing two copies in most of the cells. C: Normal retina hybridized with chromosome 18 showing two copies in all the cells. D: Breast carcinoma hybridized with chromosome 8q22 showing positive amplification (large cluster).