Figure 1. Quantification of KIF14 and E2F3 mRNA expression levels in tumor and control samples calibrated to fetal control retina (F2). There was statistically significant overexpression of KIF14 and E2F3 mRNA expression in tumors compared to fetal retinal, age-matched and adult control retinas (p<0.0001). There was a significant overexpression of E2F3 expression compared to RB cell line controls (p=0.01). The chemotherapy-treated subset (last two unshaded bars) showed a significant decrease in the expression of KIF14 (p<0.01) and E2F3 (p<0.001) compared to untreated tumors. Error bars represent standard error of the relative fold expression levels of KIF14 and E2F3.