Figure 3. RNA interference assay for IL-6 and IL-11. After administering small interfering RNAs targeting IL-6 (A) and IL-11 (B), fibroblasts were exposed to 10 ng/ml transforming growth factor (TGF)-β1 for 72 h. Then, the expression of α-smooth muscle actin (α-SMA) was evaluated using western blots. Densitimetric data represent the mean±SD of results from three independent experiments. The asterisks refer to a significant difference compared to TGF-β treatment only (p<0.001).