Figure 2. Multiplex reverse transcription PCR. Multiplex reverse transcription PCR sets for interleukins (ILs; A, B) and housekeeping genes (C). For each set of data, lane 1 represents the control group with no transforming growth factor (TGF)-β1 treatment and lane 2 represents the study group with 10 ng/ml TGF-β1 treatment for 72 h. Lane M contains positive markers. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-1RN, interleukin-1 receptor antagonist; RPL13a; SDHA, succinate dehydrogenase complex subunit A; β2M, β2-microblobulin. Two asterisks refer to a significant increase (p<0.001) in expression of IL-6 and IL-11 in the presence of TGF-β1.