Molecular Vision: Gu, Mol Vis 2009; 15:2111-2122. Table 3

Table 3. Primary antibodies used for immunocytochemistry

Antibodies	Type	Specificity in retina	Source	Dilution
Nestin	Mouse monoclonal	Progenitors, reactive glia	BD	1:200
Vimentin	Mouse monoclonal	Progenitors, reactive glia	Sigma	1:200
K_i-67	Mouse monoclonal	Proliferating cells	BD	1:200
GFAP	Mouse monoclonal	Glia	Chemicon	1:200
β3-tubulin	Mouse monoclonal	Neurons	Chemicon	1:100
Rhodopsin	Mouse monoclonal	Photoreceptors (rods)	Chemicon	1:100
PKC-α	Rabbit polyclonal	Bipolar neurons	BD	1:200

Antibodies were selected to identify expressed protein epitopes that comprised a subset of the transcripts examined by quantitative PCR. This was done to provide confirmation at the protein level of results obtained at the level of RNA. In addition, the use of immunocytochemistry allowed examination of the relative distribution and intensity of expression levels across the cultured populations and correlation of marker expression with cellular morphology. Abbreviations: GFAP represents glial fibrillary acidic protein, PKC represents protein kinase C.