Figure 7. Effect of FBS on RPC expression of markers as shown by immunocytochemistry. RPCs were grown in the absence (A, C, E, G, I, K) or presence (B, D, F, H, J, L) of FBS for seven days, fixed, and immunolabeled with antibodies against nestin (A, B), K_i-67 (C, D), β3-tubulin (E, F), GFAP (G, H), rhodopsin (I, J); and PKC-α (K, L). Final cell densities in chamber slides were dependent upon treatment conditions. Cell nuclei were counterstained with DAPI. Scale bars represent 100 μm.