Table 2 of Xu, Mol Vis 2009; 15:2094-2100. Table 2 of Xu, Mol Vis 2009; 15:2094-2100.
Table 2. Primers used for evaluating the variations by HA-SSCP or PCR-RFLP analysis of GRM6.
Variations |
Primer sequence (5’-3’) |
Product length (bp) |
Annealing temperature (°C) |
Note |
|---|---|---|---|---|
| c.68-82delAGGCGGGCCTGGCGCinsT | F-CCGAGCGCCTTCTCCCCAGGAC | 430 | 71 | |
| R-GGTGTCCCGCGAGCAGGTG | ||||
| c.726-50g>c | F-GGGCGGGACCTCTTTGTT | 214 | 68-66-64 | Touchdown PCR |
| R-CCTGGGAATCTTGATAGACTGG | ||||
| c.858-5a>g | F-GGAAGGTGCCCTAAATGC | 202 | 65 | |
| R-AAGTGGCCGGTCAGGTTGG | ||||
| c.1172G>A | F-TAGGACAGGCGAGGAACG | 102 | 70-68-66 | Touchdown PCR |
| R-TGGAGGGCGTGGGCAATG | ||||
| c.1537G>A | F-GGATCCCGGAGGCAGACG | 136 | 70-68-66 | Touchdown PCR |
| R-CCATCTTCTTCCGCTCCC |