Table 1 of Xu, Mol Vis 2009; 15:2094-2100.

Table 1. Primers used for polymerase chain reaction amplification and direct sequencing of GRM6.



Exons

Primer sequence(5’-3’)		 Product
length
(bp)		 Annealing
temperature
(°C)

Note
1 F-CCGAGCGCCTTCTCCCCAGGAC 785 68
  R-TGCGTCGGGCTCAACTGACAGG


2 F-TGTGCCCCGTCCGTGTCC 481 63
  R-TGAGGCAGGGCTGAGTCGTC


3 F-GGGCGGGACCTCTTTGTT 521 65
  R-GCTATTCAGTCTGGGCTTGTG


4~7 F-CCGGCCCCGTTTTTCCTG 4132 69 LA Taq DNA Polymerase
  R-CACCTGGGACGCACAAAACAC


  F1-GCCATGCCTAGTTCACCT / / Sequencing primer
  F2-ATTTGCACGTCCCTTATGAGC

Sequencing primer
  R1-CTTTCCTCGCACGCCATCC / / Sequencing primer
  R2-TCAGCCTCACCCAGCCCTTCC

Sequencing primer
8 F-TCAACGAGAACGGAGATGC 900 61
  R-CCCTTTTGGCTTTGTAACG


9 F-CTGAGAGCCACCACGAAGAG 567 65
  R-GCAGCCAGATACGGGATAAA


10 F-GGGCCCCTGCTCATACTGTT 460 65
  R-CTCCCTGCCACTGACTGTTC


In the "Primer sequence" column, "F" indicates the forward primer and "R" indicates the reverse primer. GC1 buffer (Takara Biotechnology Dalian CO. Ltd, Liaoning, China) is designed for amplification of templates having complex secondary structure or high GC content. LA Taq DNA Polymerase (Takara Biotechnology Dalian CO. Ltd, Liaoning, China) was designed for amplification of long targets. The amplicon from exon 4 to 7 was sequenced with the additional primers listed as F1, F2, R1 and R2.

Xu, Mol Vis 2009; 15:2094-2100. http://www.molvis.org/molvis/v15/a225

©2009 Molecular Vision http://www.molvis.org/molvis/

ISSN 1090-0535