Figure 3 of Barbaro, Mol Vis 2009; 15:2084-2093.


Figure 3. Keratocytes in the stromal part of the hemicornea were observed after 4',6-diamidino-2-phenylindole (DAPI) staining (A). Expression of specific markers of the corneal stroma was analyzed: keratan sulfate (KS), essential for the maintenance of the correct orientation of collagen fibrils (B); CD-34, a specific marker of keratocytes expressed both in vivo and in vitro (C); ALDH3A1, a marker of quiescent keratocytes in vivo (D); VSX1, found in keratocytes undergoing fibroblastic transformation (E); α-SMA, a muscle protein of F-actin stress fibers, typically found in myofibroblasts (F). Scale bar=50 μm.