Molecular Vision: Abouzeid, Mol Vis 2009; 15:2074-2083. Table 2

Table 2. List of initial buffer B concentrations and temperatures for each DNA fragment.

PCR				WAVE
Exon	Sense primer (5’-3’)	Antisense primer (5’-3’)	Annealing temperature °C	Temperature °C	% start B
1	TGTTGCGGAGTGATTAGTGG	TCCTGGGAAGGAGACAGAGA	60 +betain	60.8	57.9
2	ACACACTTGAGCCATCACCA	CTCCTGCGTGGAAACTTCT	60 +betain	59.3	60.6
3	GTGGGTGTAATGCTGGGACT	CCCAATCTGTTTCCCCTACA	60 +betain	56	59.7
4	CCCCAAGAGGTTGAGTGGAT	GTCGCGAGTCCCTGTGTC	60 +betain	61.4	57.1
5	TGAGGATGCATTGTGGTTGT	GTGGAAGGAGAGGGGAAAGT	60 +betain	59.5	60.8
6	TTCAGGCAGTGTTTAAGAAAAGTT	ACTCACACATCCGTTGGACA	55	54.3	58.7
7	TGCAGATGCAAAAGTCCAAG	CTCTGTTCCCCCAGGTACAA	60 +betain	57.4	61
8	TTTCCACGGTGTATCTGCAA	AAGCCCTGAGAGGAAATGGT	60 +betain	59.6	59.9
9	ACCTTGGGAATGTTTTGGTG	CACTGAAAAGATGCCCAGAGA	60 +betain	56.7	60.3
10	AGGTGGGAACCAGTTTGATG	CATGGCAGCAGAGCATTTAG	60 +betain	57.5	58
11	TTCAGTCTGCTAAATGCTCTGC	TGTGAGGGCTGTGTCTGTTC	60 +betain	59	60
12	ACCACACCGGGTAATTTGAA	CTCTCAAGGGTGCAGACACA	60 +betain	58.7	58.4
13	TAGCTCGAGGCCCAATCTTA	TAAACACGCCCTCCCATAAG	60 +betain	58.3	60
14	TTTCTGAAGGTGCTACTTTTATTTG	AAGTCCATTCCTTCCCCAGT	55	53.5	60.2
15	AAACTTAAGTGTTTTGAAGTTGTTCAC	CCCAGATTGAAAATGCCAGT	60 +betain	54.3	60.5