Figure 5. Representative reverse transcription-PCR analysis for ETX1 in normal and glaucomatous trabecular meshwork tissues. RNA was isolated from human TM lysates, using the Trizol method, and cDNA was prepared using established protocols, as described in the methods section. The PCR products were then run on a 2% agarose gel at 100 V for 80 min. The expected size of ETX1 is approximately 220 bp.