Figure 2. Localization of expression of ETX1 in human retina by immunofluorescence. A: Merged image of ETX1 and DAPI in normal retina. Retinal ganglion cell (RGC) layer is intact in the normal retina with good cellularity. B: Normal retina stained with anti-ETX1 and secondary antichicken antibody coupled with Cy5. C: Merged image of ETX1 and DAPI in glaucomatous retina. RGC layer shows decreased cellularity compared to the normal. D: Staining for ETX1 in glaucomatous retina. Clusters of ETX1 indicated with arrows are more frequent and cover more swaths of area in glaucomatous tissue in both the RGC layer as well as diffusely throughout the retina. RGC: Retinal ganglion cell; INL: Inner nuclear layer; ONL: Outer nuclear layer.