Figure 4. Cu2+-induced
self-aggregation of αA-crystallin, G98R αA-crystallin, and their mixed
oligomer. Aggregation of 0.1 mg/ml of αA-crystallin (○), G98R
αA-crystallin (●), and their mixed oligomer (◓) as a function of
increasing Cu2+ concentration at 37 °C in buffer B was
monitored by light scattering at 465 nm expressed in arbitrary units
(AU). G98R αA-crystallin and its mixed oligomer with wild type protein
exhibit increased vulnerability to Cu2+-induced
self-aggregation.