Table 1 of Wang, Mol Vis 2009; 15:2045-2049. Table 1 of Wang, Mol Vis 2009; 15:2045-2049.
Table 1. Primers used for the amplification and sequencing of OPTC and EPYC.
Gene |
Exon |
Direction |
Primer sequence |
Size of PCR product (bp) |
Annealing temperature (°C) |
|---|---|---|---|---|---|
| OPTC | E2 | F | 5'-CGAGCCATTCCCACAACACT-3' | 439 | 64 |
| R | 5'-GCTGGAGACACCCGACTTT-3' | ||||
| E3 | F | 5'-TTCCCTCCTCCTCCTCCACA-3' | 387 | 62 | |
| R | 5'-GTAGCCCTGACCCTCCAT-3' | ||||
| E4 | F | 5'-TGTACATGGTGCCCGTCAGT-3' | 425 | 62 | |
| R | 5'-GAGAGCCAACAGGAAAGAGC-3' | ||||
| E5 | F | 5'-AGGTGGAGAGCTGGTTAGAA-3' | 433 | 64 | |
| R | 5'-GGTGGTGGTGGAGGTGAGTG-3' | ||||
| E6 | F | 5'-GCGCATGGCTGAGGCTAAG-3' | 346 | 62 | |
| R | 5'-CAATCAAGGAGACCCCACAG-3' | ||||
| E7 | F | 5'-TTGGCCCTAGATGCTTCAGT-3' | 425 | 62 | |
| R | 5'-ATTAATGCCTGGGCTAAAGA-3' | ||||
| SSCP | F | 5'-CTGCGTGTGCCTCGGTTCCTCT-3' | 180 | 64 | |
| R | 5'-ATGCCTGCCCCCACTCCTCTTT-3' | ||||
| EPYC | E2 | F | 5'-ACATCTGGCAGCAATGGTC-3' | 538 | 60 |
| R | 5'-TCCGGTATTAACCTCCAAAAG-3' | ||||
| E3 | F | 5'-CAAGCAGGATGGTCAAACTAT-3' | 451 | 62 | |
| R | 5'-TGCTTCAAACCTAGCCTATTC-3' | ||||
| E4 | F | 5'-CTTGGTTAGTGGGAGACTCTA-3' | 371 | 62 | |
| R | 5'-AACCAGGCTTTGGCATTCAA-3' | ||||
| E5-1* | F | 5'-ATTAACTGTGCATTGCTAAAT-3' | 473 | 44-68 | |
| R | 5'-AAGCCTGGGTGACAGAGTGA-3' | ||||
| E5-2* | F | 5'-TAATGCCTGTATCTTTGACTAT-3' | 630 | 44-68 | |
| R | 5'-AATTTATTTTCCCTACACGA-3' | ||||
| E5-3* | F | 5'-GTTGCTTTATGATGGATGAATG-3' | 979 | 44-46 | |
| R | 5'-CCTAGGAAATATGGCGAAACC-3' | ||||
| E5-4 [25] | F | 5'-CGTGGTGTACCAATTTCTTTGCT-3' | 579 | 44-68 | |
| R | 5'-ACTCAAGCCTGGGTGACAGAGTG-3' | ||||
| E6 | F | 5'-AAAACTAAGCTGCCTCAACT-3' | 353 | 60 | |
| R | 5'-AACATGCCATTACAGACAAA-3' | ||||
| E7 | F | 5'-TTACCCCCATTGCTTAGAAA-3' | 433 | 60 | |
| R | 5'-GAACACAATCTCAAAGTATCA-3' |