Figure 7. Effects of EGF on caveolin-1 expression and apoptosis in HLE-B3 cells treated with high concentrations of glucose. Cells were incubated in DMEM containing both glucose and EGF at the indicated concentrations for 48 h. The ∆C_t values of CAV1 mRNA levels (A) and the percentages of apoptotic cells (B) were determined by real-time RT–PCR and flow cytometry, respectively. Experiments were performed in triplicate. A: The asterisk indicates p<0.01, and the hash mark denotes p>0.05. B: The asterisk indicates p<0.05, and the hash mark denotes p>0.05.