Figure 5. Transmission electron microscopy of caveolae in HLE-B3 cells treated with high concentrations of glucose. Cells were seeded on glass coverslips, treated with 5 mM or 25 mM glucose for 48 h, fixed with glutaraldehyde, post-fixed with osmium tetroxide, and stained with uranyl acetate and lead citrate. A: 5 mM glucose-treated (control) cells. Arrows point to caveolae. B: 25 mM glucose-treated cells.