Figure 4. Subcellular distribution of caveolin-1 and externalization of phosphatidylserine in HLE-B3 cells treated with high concentrations of glucose. HLE-B3 cells were treated with 5 mM glucose (A, C) or 25 mM glucose (B, D, E, F) for 48 h and then reacted with anti-caveolin-1 polyclonal primary antibody (1:200; A, B, E) or Alexa Fluor 488-conjugated annexin V (C, D, F). Cells were examined with an immunofluorescent microscope. G is a merged image of E and F, indicating colocalization of caveolin-1 and PS in the cell membrane. The scale bars indicate 15 μm.