Figure 3. Western blot analysis of caveolin-1 in HLE-B3 cells treated with high concentrations of glucose. Cells were treated with the indicated concentrations of glucose in DMEM for 48 h (A) or 25 mM glucose in DMEM for 1–48 h (B). Cells treated with 5 mM glucose and 20 mM mannitol were used as an osmotic control. The experiments were performed in triplicate.