Figure 1 of Zhang, Mol Vis 2009; 15:2008-2017.

Figure 1. Effects of high concentrations of glucose on apoptosis of HLE-B3 cells. Cells were incubated with 5–25 mM glucose for 48 h (A) or with 25 mM glucose for 1–48 h (B). Cells treated with 5 mM glucose and 20 mM mannitol were used as an osmotic control. Apoptosis was determined by using the Vybrantan® apoptosis assay kit #2 and by flow cytometry. Experiments were performed in triplicate. The asterisk indicates p<0.05 versus control, and the hash mark denotes p>0.05 versus control