Figure 1. Effects of high concentrations
of glucose on apoptosis of HLE-B3 cells. Cells were incubated with 5–25
mM glucose for 48 h (A) or with 25 mM glucose for 1–48 h (B).
Cells treated with 5 mM glucose and 20 mM mannitol were used as an
osmotic control. Apoptosis was determined by using the Vybrantan®
apoptosis assay kit #2 and by flow cytometry. Experiments were
performed in triplicate. The asterisk indicates p<0.05 versus
control, and the hash mark denotes p>0.05 versus control