Figure 3. Examination of IκBα mRNA and protein levels in HCM cells 24 h, 48 h, and 72 h after IκBα siRNA transfection. A: IκBα mRNA expression was quantified by real-time RT–PCR. Expression levels were normalized with GAPDH. Error bars represent standard deviations (SD) calculated from three parallel experiments. B: Total cell lysates from HCM cells treated with IκBα siRNA, nonsense control siRNA (NC), and control were analyzed by western blot with IκBα antibody and GAPDH antibody. The arrows indicate IκBα (39 kDa) and GAPDH (36 kDa) bands. The bands were analyzed densitometrically, and the values were normalized with GAPDH, which are represented in the bar graph. The mRNA and protein values of IκBα siRNA, control, and nonsense control siRNA (NC) groups were determined by one-way ANOVA. The double asterisk denotes p<0.01.