Figure 2. Transfection efficiency of IκBα siRNA. To monitor siRNA transfection efficiency, the HCM cells were transfected with Cy3-labled IκBα siRNA using Lipofectamine 2000 reagent, which were viewed at 24 h, 48 h, and 72 h after transfection. Cy3-labled IκBα siRNA (red) was observed in the cytoplasm in HCM cells, and the transfection efficiency was 92% (A), 90% (B), and 91% (C) 24 h, 48 h, and 72 h after IκBα siRNA transfection, respectively.