Figure 1. Identification of HCM cells. The third passaged HCM cells were washed with PBS, fixed with formaldehyde, blocked in BSA/PBS and stained with monoclonal anti-smooth muscle α-actin and rhodamine-conjugated goat anti-rabbit IgG;HCM cells were also stained with monoclonal rabbit anti-Desmin and goat anti-rabbit IgG. HCM cells labeled with antibody to smooth muscle actin (A; red) and the negative control (B). Nuclei staining with DAPI is shown in blue. HCM cells labeled with antibody to desmin (Buffy; C) and the negative control (D).