Figure 8. Effect of PSP on HUVEC tube formation. HUVECs were plated on the surface of Matrigel in complete media with 10, 50, or 100 µg/ml of PSP or without PSP, and tube formation was evaluated 6 h later. The angiogenic stuctures was inhibited by PSP obviously especially above concentration of 50 µg/ml. One representative experiment of three separate assays is shown.