Figure 3. Effect of blue light on MMP. A: Representative images of JC-1 labeled mitochondria of ARPE-19 cells. Unirradiated cells (control) and irradiated cells (1 mW/cm²) were stained after 3 h, 24 h, or 72 h. Each panel is representative of 8 separate experiments (Bar=50 μm). B: Statistical analysis of JC-1 labeled mitochondria of ARPE-19 cells. Unirradiated cells (control) and irradiated cells (1 mW/cm²) were stained after 3 h, 24 h, or 72 h. 40 images from n=8 separate experiments were taken and red and green fluorescences of JC-1 were counted to quantify MMP. Blue light treatment for 24 h resulted in a significant decrease in red-green ratio of JC-1. The asterisk (*) indicates a p<0.05 (one-way ANOVA and Bonferroni test).