Table 1 of Guduric-Fuchs, Mol Vis 2009; 15:1915-1928.

Table 1. Primary antibodies used for immunohistochemical analysis of the pig retina.

Antibody Cell specificity Host Dilution Source Reference
Nestin neural stem/progenitor cells, Müller glia mouse 1:400 BD Biosciences [25,41,44]
Pax6 retinal progenitors, ganglion, amacrine, and horizontal cells rabbit 1:1,000 Chemicon [28,45]
Ki67 proliferating cells mouse 1:300 BD Biosciences [46]
Brn3a ganglion cells mouse 1:40 Chemicon [47]
Neurofilament (NF)-160 ganglion, horizontal, and bipolar cells mouse 1:350 Sigma [22,38,48,49]
AP2α amacrine cells mouse 1:50 Developmental Studies Hybridoma Bank [26]
Calretinin ganglion, amacrine, horizontal, cone photoreceptor, and bipolar cells mouse 1:500 Chemicon [33,50]
Calbindin horizontal, ganglion, amacrine, bipolar, and cone photoreceptor cells rabbit 1:1,500 Chemicon [27,51,52]
GAD65 GABAergic amacrine, rare horizontal ganglion cells mouse 1:1,000 Developmental Studies Hybridoma Bank [27,28]
Islet1 amacrine, bipolar, ganglion, and horizontal cells mouse 1:500 Developmental Studies Hybridoma Bank [29,37]
PKCα rod bipolar, amacrine, and ganglion cells, cones mouse 1:400 Sigma [27,30,53]
Recoverin photoreceptors, cone bipolar cells, rare ganglion cells rabbit 1:1,000 K. Koch [42,51,54]
Rhodopsin (Rho4D2) rods mouse 1:100 R. Molday [2,36,43]
GNAT2 cones rabbit 1:500 Santa Cruz Biotechnology [55-57]
P75 neurotrophin receptor Müller glia and ganglion cells rabbit 1:350 Promega [31,32,58]
Glutamine synthetase Müller glia mouse 1:500 BD Biosciences [35,59]
GFAP astrocytes rabbit 1:500 DAKO [60-62]

The cell specificity is described according to the published literature (listed in the reference column) on the antibodies to the same proteins. Details for the primary antibodies (host, dilution used and the source) used in our study are presented.

Guduric-Fuchs, Mol Vis 2009; 15:1915-1928. http://www.molvis.org/molvis/v15/a204

©2009 Molecular Vision http://www.molvis.org/molvis/

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